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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology |
| Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Blood group systems and antigens - Transfusion reactions and adverse events - Blood components and storage - Antibody detection and identification |
| Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis |
| Hematology | 20-25% | - White blood cell morphology and diseases - Routine and automated hematology procedures - Hemostasis and coagulation testing - Red blood cell physiology and disorders |
| Immunology and Serology | 5-10% | - Immunologic testing methodologies - Infectious disease serology - Autoimmune and immunodeficiency disorders - Immune system principles and components |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. The A and B antigens are present on the red cells of an AB patient. H antigen is a precursor to the ABO antigens.
An individual with type AB blood will demonstrate the complete absence of which of the following antigen sites?
A) None of the above
B) H
C) B
D) A
2. The activities conducted in a laboratory with a certificate of registration include:
A) waived tests only
B) moderate- or high-complexity lab testing until the lab is determined by survey to be in compliance
C) physicians to perform microscopy only
D) urinalysis only
3. Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by intravascular hemolysis, is one of the most common symptoms associated with this condition.
Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?
A) Levodopa
B) Cephalosporins
C) Levofloxacin
D) Nonsteroidal anti-inflammatory drugs (NSAIDs)
E) Quinidine
4. Entamoeba gingivalis resembles Entamoeba histolytica both in size and in nuclear characteristics. Entamoeba gingivalis may contain numerous cytoplasmic inclusions such as red blood cells, white blood cells, and bacteria.
I reside in the mouth where I measure approximately 17 micro meters.
A) Entamoeba gingivalis trophozoite
B) Entamoeba coli trophozoite
C) Acanthamoeba species trophozoite
D) Trichomonas vaginalis trophozoite
5. On the red cell membrane, there is a bicarbonate / chloride exchanger. This exchanger allows for bicarbonate to leave the red cells while chloride is allowed inside.
In blood, bicarbonate leaves the red blood cell and enters the plasma through an exchange mechanism with:
A) chloride
B) sodium
C) potassium
D) phosphorus
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: B | Question # 3 Answer: B | Question # 4 Answer: A | Question # 5 Answer: A |


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